Updating the rna polymerase ctd questions to ask a guy you are dating
RNA polymerase II generates all protein-coding m RNAs as well as a large number of non-coding micro RNAs (mi RNAs), small nuclear RNAs (sn RNAs) and small nucleolar RNAs (sno RNAs).The enzyme is composed of 12 subunits (RPB1–12), five of which are shared among the three eukaryotic polymerase complexes.Given the association between polymerase pausing and ac RPB1, the potential to acetylate these residues may have enabled tighter control of gene expression as animals grew in complexity and diversified.Indeed, ac RPB1 now influences the regulation of growth factor-target genes and genes involved in lineage-specific processes, such as cell adhesion and vasculature development, in mammals.). The consensus CTD repeat motif and tandem organization represent the ancestral state of eukaryotic RPB1, but across eukaryotes CTDs show considerable diversity in repeat organization and sequence content.These differences may reflect lineage-specific CTD functions mediated by protein interactions.The largest subunit, called RPB1, is unique to RNA polymerase II and is involved in its catalytic activity.
Ancestral counts were inferred for each internal node of the tree using symmetric Wagner parsimony.
Conserved heptad repeats are found in the linker-proximal part of the mammalian CTD, but the sequence of the distal heptad repeats, which are not present in yeast, diverge from this consensus sequence.
Eight of the non-consensus repeats in human and mouse CTDs carry a lysine at position 7 (K The human RNA polymerase II subunit 1 (RPB1) C-terminal domain (CTD) contains more heptad repeats than the yeasts, and eight of its non-consensus distal repeats have a lysine residue.
However, in spite of our increasing knowledge of these marks, the full number and identity of repeats that undergo modification during transcription are unknown.
The CTD repeat motif and tandem repeat orientation are observed in most eukaryotic model organisms, and this configuration evolved early in the history of eukaryotes .